Gobal fitting
Posted by paulbarber on 2011-11-18 11:57
Just to re-iterate...
The global fit can be used in any situation where you want to find the dominant average lifetime(s) in an image but also want to see what areas the species occupy. A bi-exp global assumes that there are 2 dominant lifetimes. This is useful for FRET with a bio-sensor which is probably only in an open or closed configuration, and nothing in between. If there is a great variation in distance between donor and acceptor, a global fit is not suitable. A global fit is much more robust than bi-exponential pixel fits which are plagued by different problems. This was discussed a bit in our J R Soc Interface paper from 2009 (attached).
Cheers,
The global fit can be used in any situation where you want to find the dominant average lifetime(s) in an image but also want to see what areas the species occupy. A bi-exp global assumes that there are 2 dominant lifetimes. This is useful for FRET with a bio-sensor which is probably only in an open or closed configuration, and nothing in between. If there is a great variation in distance between donor and acceptor, a global fit is not suitable. A global fit is much more robust than bi-exponential pixel fits which are plagued by different problems. This was discussed a bit in our J R Soc Interface paper from 2009 (attached).
Cheers,
Barber_2009.pdf | 756 KB |
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